R-Chaetoceros Neogracile Polypeptide-1 is a single chain recombinant protein produced by fermentation in E. coli. The starting gene, which codes for Antifreeze protein, is directly isolated from Chaetoceros neogracile. The protein contains a maximum of 282 amino acids which may contain disulfide bonds and/or glycosylation, and consists of the proper sequence of the 20 standard amino acids.