Defined Cell Culture Media 20 is a cell culture mixture consisting of Adenosine, Alanine, Arginine, Ascorbic Acid, Asparagine, Aspartic Acid, Biotin, Calcium Chloride, Calcium Pantothenate, Choline Chloride, Cyanocobalamin, Cysteine HCl , Cytidine, 2'-Deoxyadenosine, Deoxyguanosine, Deoxycytidine, Folic Acid, Glucose, Glutamic Acid, Glutamine, Glycine, Guanosine, Histidine, Inositol, Isoleucine, Leucine, lipoic acid, Lysine, Magnesium Sulfate, Methionine, Niacinamide, Potassium Chloride, Proline, Pyridoxine HCl, Riboflavin, Serine, Sodium Bicarbonate, Sodium Chloride, Sodium Phosphate, Sodium Pyruvate, Thiamine HCl, Threonine, Thymidine, Tryptophan, Tyrosine, uradine, and {5411; Valine}.